Type
Text
Type
Thesis
Advisor
Sirotkin, Howard I | Martin, Benjamin L
Date
2012-12-01
Keywords
Biochemistry--Cellular biology--Developmental biology | Activin Response Element, Gene Targeting, Nodal
Department
Department of Biochemistry and Cell Biology
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/71346
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Use of zinc finger nucleases is an established method for gene targeting in zebrafish. Such strategies are efficient in targeting a single gene of interest. Our study attempts to target many Nodal related genes by targeting the Activin Response Element (ARE) through the use of native DNA binding domains tethered to the Fok1 endonuclease. In this light, genes are targeted in a Nodal-dependent manner, and can retain transcript levels sustained by other signaling pathways. This technique holds the potential to be a high throughput means of generating mutations in the regulatory regions of various Nodal responsive genes with the use of one set of constructs. | 59 pages
Recommended Citation
Minder, Jessica Lauren, "Use of Native DNA Binding Domains Tethered to the Fok1 Endonuclease to Target Nodal Related Genes via the Activin Response Element" (2012). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 552.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/552