Type
Text
Type
Dissertation
Advisor
Advisors: Pan, Yingtian; Jia, Shu; Du, Congwu; Tsirka, Styliani-Anna (Stella) E.
Date
2017-12-01
Keywords
Biomedical engineering, Cocaine, Neurosciences, Microvascular dynamics, Optics, Optical coherence tomography, vasoconstriction
Department
Department of Biomedical Engineering
Language
en
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/78318
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Optical domain technologies can provide high spatiotemporal resolution for biomedical research and therefore attract tremendous interests in multiple disciplines including psychiatry, neurology, ophthalmology, oncology, urology and many more. Optical coherence tomography (OCT), after more than 20 years of development, has been becoming one of the most widely-used bio-optical modalities not only for laboratory-based research but also for clinical applications. For example, OCT becomes the standard of care for diagnosing glaucoma. Additionally, its derived velocimetry, optical coherence Doppler tomography (ODT), also demonstrates the unique capability of mapping cerebral blood flow velocity (CBFv) of vascular networks with capillary resolution (&phis;<6µm) and thus allows for the widespread utilization in numerous neurology studies. Although ODT has been widely used in biomedical research, there remain several technical limitations including the shallow penetration depth, low sensitivity for imaging slow flow in capillary and the measurement error due to Doppler angle. To address these challenges, viable solutions are proposed in this dissertation. Chapter 1 starts with a comprehensive review over basic principle of OCT and ODT. Chapter 2-4 explicitly address these OCT technical challenges, for instance, using a 1310nm super-luminescent Diode (SLD) laser source to extend optical penetration depth, utilizing optical contrast agent: intralipid to enhance ODT’s sensitivity for capillary flow detection, implementing numerical methods to extend ODT’s dynamic range and decouple Doppler angle. In addition to these technical improvements, Chapter 5-6 address two biomedical challenges in oncology and psychiatry. Specifically, Chapter 5 introduces a longitudinal optical access to rodent cerebral vasculature and a method of tumor boundary detection, which provides a powerful insight into the tumor progression. Chapter 6 reveals the close correlation between cocaine-elicited transient ischemic attack (TIA) and hypo-perfused cortex. Chapter 7 focuses on the development of multi-modality system and novel imaging scheme for the study of neurovascular coupling. Chapter 8 briefly concludes the contents presented in this dissertation and outlines the future development and potential applications of ODT. | 129 pages
Recommended Citation
You, Jiang, "Quantitative imaging of 3D cerebral microvascular network dynamics by ultrahigh -resolution optical coherence Doppler tomography (μODT)" (2017). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 3812.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/3812