Type
Text
Type
Thesis
Advisor
Advisors: Wollmuth, Lonnie P; Hsieh, Helen
Date
2017-12-01
Keywords
Neurosciences, Biochemistry, Cytology
Department
Department of Biochemistry and Cell Biology | Thesis
Language
en
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/78298
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
AMPARs play an essential role in fast excitatory neurotransmission. Before native AMPARs can function at the synapse they must be processed intracellularly. These ion channels are synthesized and assembled into multimeric complexes, shuttled in a regulated fashion between intracellular compartments and are finally inserted into the membrane to carry out their function. Important binding partners known as auxiliary subunits associate with AMPARs at the synapse, but also during assembly and trafficking. The presence of auxiliary subunits in AMPAR complexes at the membrane has been demonstrated through electrophysiological studies, showing effects on the gating and pharmacology of AMPARs. The localization of AMPAR-auxiliary subunits complexes in cellular compartments has not been well characterized, and the stoichiometry of these auxiliary subunits for appropriate function is not known. The main objective is to further characterize auxiliary subunits beyond their effects on AMPAR gating at the synapse. Cerebellar Granule Neurons were to be used as the native environment to study AMPAR-auxiliary complexes to address both the localization of these complexes and the stoichiometry of auxiliary subunits. The prediction is that auxiliary subunits complex with AMPARs in a compartment-specific manner and this allows for the appropriate processing of AMPARs. | 35 pages
Recommended Citation
Certain, Noele Doreen, "The Intracellular Cell Biology of Ionotropic Glutamate Receptors" (2017). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 3792.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/3792