Type
Text
Type
Thesis
Advisor
Goroff, Nancy | Drueckhammer, Dale G | Koch, Stephen.
Date
2015-08-01
Keywords
Chemistry
Department
Department of Chemistry.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/77111
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Ocean acidification has been shown to endanger shelled marine organisms, since increased CO2 intake by oceans will decrease pH of seawater and enhance Ca2+ dissolution from shell skeletons. Determination of Ca2+ concentration in the marine environment would not only help to provide information about local calcium ion distribution and activities, but also provide a sketch of the living condition of shelled marine organisms. A number of optical sensors for calcium ion detection have been developed. However, these detectors were mostly designed for intracellular detections or relatively low ion concentration conditions (10− 6 to 10− 8 M) and have high calcium affinity, which is not suitable to apply in a marine environment where calcium concentration is much higher (∼ 10mM). The objective of this project is to develop a fluorescence-based calcium sensor with low calcium affinity for calcium distribution imaging in marine environments. The synthesis of this sensor is the first step towards the development of an optical instrument for direct in situ, non-destructive and high-resolution two dimensional imaging of Ca2+. The experimental approach involves the coupling of a fluorophore with a calcium binding ligand. When binding to calcium ions, the fluorescence of the sensor will increase and hence shows correlation to calcium concentration in the sample. Further steps include verification of the coupling product, examination of the calcium ion binding ability, and possibly the application in artificial marine settings. | 65 pages
Recommended Citation
Huang, Jingyu, "Synthesis of a Fluorescent Sensor for Calcium Measurement in Marine Sediments" (2015). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2948.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2948