Type
Text
Type
Dissertation
Advisor
Pan, Yingtian | Liu, Jonathan T.C. | Entcheva, Emilia | Weinacht, Thomas.
Date
2014-12-01
Keywords
Biomedical engineering | Confocal microscopy, Fluorescence imaging, In vivo imaging, Optical design, Phantom design
Department
Department of Biomedical Engineering.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/77001
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
The prevalence of oral cancer has been increasing at an alarming rate in recent years. Due to a limited understanding of the cause and progression of this disease, patient prognosis remain poor, and the mortality and morbidity of the disease remain significant. The need to diagnose cancerous regions early is key to patient survival. However, the high cost and invasive nature of standard pathological analysis motivates the search for an alternative real-time imaging technology that provides accurate non-invasive early diagnosis of the disease. The goal of this thesis is to develop a line-scanned dual-axis confocal (LS-DAC) microscope for high-resolution, high-contrast, high-speed imaging suitable for real-time non-invasive clinical diagnoses. The performance of the LS-DAC microscope architecture is characterized by measuring its axial and lateral response in water as well as a homogeneous scattering phantom (Intralipid). The accuracy of the results are assessed and confirmed by comparison with results from a Monte Carlo scattering simulation of the system. In addition, a parallel comparison between the LS-DAC and our existing point-scanned dual-axis confocal (PS-DAC) microscope is performed to ensure comparable imaging quality at shallow depths. Furthermore, a heterogeneous phantom has been developed and a deconvolution algorithm utilized to aid the characterization and contrast enhancement of the DAC setup, respectively. Finally, the performance of the LS-DAC is demonstrated by imaging ex vivo and in vivo biological samples in fluorescence mode. The tabletop LS-DAC developed in this thesis research serves as a basic prototype and benchmark system for a miniaturized portable LS-DAC that is also being developed in our lab for affordable non-invasive, real-time diagnostic purposes in patients. | 68 pages
Recommended Citation
Wang, Danni, "Development of a Line-scanned Dual-axis Confocal Microscope for Video-rate Diagnostic Imaging" (2014). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2865.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2865