Type
Text
Type
Thesis
Advisor
Sternglanz, Rolf. | Hollingsworth, Nancy
Date
2014-12-01
Keywords
Cellular biology
Department
Department of Biochemistry and Cell Biology.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76924
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Recombination is an essential meiotic process to ensure proper chromosome distribution. Recombination is promoted by the synaptonemal complex, a structure which bridges two pairs of homologous sister chromatids, bringing them into close proximity. The synaptonemal complex is formed by condensation of the sister chromatids on protein cores called axial elements. Homologous axial elements are then held together in budding yeast by a protein called Zip1. Zip1 is part of the ZMM group of proteins that work together during meiosis to promote the formation of crossovers which are distributed throughout the genome by interference. Four phosphorylation sites have been identified in the Zip1 C-terminus that are essential for the formation of interfering crossovers. To identify potential binding partners with the phosphorylated Zip1 C-terminus a two-hybrid screen was conducted using a zip1 mutant containing phosphomimetic amino acid substitutions. In this thesis I report the identification of three novel protein-protein interactions with the negatively charged Zip1 C-terminus. | 59 pages
Recommended Citation
Murray, Matthew, "Identification of binding partners with the negatively charged Zip1 C-terminus using a two-hybrid screen" (2014). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2797.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2797