Type
Text
Type
Thesis
Advisor
Cao, Jian | Mao, Cungui.
Date
2016-12-01
Keywords
Molecular biology
Department
Department of Biochemistry and Cell Biology
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76900
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Breast cancer is the most common cancer in women and metastasis is the primary cause for mortality. Matrix metalloproteinases (MMPs) play important roles in cancer cell migration and invasion. Tumor suppressor gene TP53 is the most frequently mutated gene in cancer, and p53 mutation occurs in invasive breast cancer with higher frequency than in non-invasive breast cancer. However, the nature of relationship between p53 and MMPs remains inconclusive. Here we show that wild type p53 could repress transcriptional activity of MMP-9 and MMP-14, while the DNA-contact mutant p53 R280K could upregulate the transcription of MMP-9 and MMP-14. This regulation might be Sp1-dependent as there is a p53/Sp1 overlapping binding site on the promoters of MMP-9 and MMP-14. Although additional study is needed to further confirm this mechanism, our finding would provide a potential target for anti-metastatic therapy. | 29 pages
Recommended Citation
Xue, Yingjiao, "Transcriptional Regulation of MMP-9 and MMP-14 by p53 and its Mutant R280K" (2016). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2774.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2774