Type
Text
Type
Dissertation
Advisor
Gersappe, Dilip | Rafailovich, Miriam H | Jones, Keith | Simon, Marcia.
Date
2015-05-01
Keywords
dental pulp stem cell, fibrinogen, platelets, polymer substrate, thrombosis, tooth regeneration | Materials Science
Department
Department of Materials Science and Engineering.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76283
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Choosing the right materials can be critical to biomedical device performance. Usually factors such as biocompatibility, function, and cost are considered. Here we focused on the interactions between proteins, cells, and the material surfaces used for their biomedical application. The influence of surface hydrophobicity and hydrophilicity on adsorbed fibrinogen conformation, orientation, fiber formation and platelet adhesion has been investigated to understand the interactions between blood components and the materials used in blood-contacting medical devices. The results indicate that fibrinogen adsorbed to either hydrophobic or hydrophilic polymer surfaces binds platelets, implying that both surfaces are potentially thrombogenic. We present a model for surface initiation of clots, which enabled the design of a quick screen for thrombogenesis and guides the innovation and design of new anti-thrombogenic materials. In addition, the interactions of polyisoprene (PI) and commercially Gutta-percha (nanoparticles filled PI) with dental pulp stem cells (DPSC) has been evaluated. The composition of three different kinds of Gutta-percha is consistent with their mechanical properties. The biocompatibility tests show that the Gutta-percha used in our study are non-cytotxic for human adult DPSCs, and could induce biomineralization by DPSCs without the addition of a chemical inducer. Then, the study of influence of PI substrates moduli on differentiation of DPSCs show that the “hard, G>2.3MPa†substrates can improve cell proliferation and induce biomineralization without chemical inducer. The results indicate that Gutta-percha, which is a filling material currently used in endodontic practice, can be potentially used in regeneration of the tooth, rather than obduration of the canal. PI as a polymer matrix can also be used in re-engineer scaffold for tooth regeneration therapy. Hence, the study of interface between protein/cells and substrates could help the choice of materials and the design of biomedical devices. | 108 pages
Recommended Citation
Zhang, Liudi, "The Role of Protein/Substrate Interface in the Functioning Biomedical Devices" (2015). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2209.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2209