Type
Text
Type
Dissertation
Advisor
Du, Guangwei | Joav Prives | Deborah A. Brown | Michael A. Frohman.
Date
2010-08-01
Keywords
Health Sciences, Pharmacology | actin reorganization, Lipid Signaling, phosphatidic acid, PIPKI
Department
Department of Molecular and Cellular Pharmacology
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/72651
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Production and breakdown of certain phosphoinositides plays key roles in a variety of cellular processes. Among these, phosphatidylinositol-4, 5-phosphate (PIP 2) is well known for its critical functions in membrane trafficking, cytoskeletal organization, and signal transduction. Type I phosphatidylinositol-4-phosphate 5-kinase (PIPKI) is the main enzyme responsible for the synthesis of PIP2. Although PIPKI activity is regulated by small G proteins and phosphatidic acid (PA), the contribution of these upstream regulators in actin cytoskeletal reorganization remains unclear. The binding region of some other PA-regulated proteins has been identified. From these, it appears that binding may either be direct or electrostatic in nature. I have identified basic residues in the proposed membrane-binding region of PIPKIΓ that are required for membrane translocation, actin reorganization, and stimulation by PA. I also demonstrate here that the direct binding of PIPKI to PA through these residues is required for these important functions.
Recommended Citation
Roach, Akua Ntiamoah, "The Role of Phosphatidic Acid in PIPKI-regulated Actin Reorganization" (2010). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 1854.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/1854