Type
Text
Type
Thesis
Date
2007-12-01
Keywords
metastasis | ovarian cancer | tumor cells
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/70792
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Metastasis is a common cause of cancer death. Tumor cells metastasized in the peritoneal organs are associated with formation of ascites in patients with advanced stages of epithelial ovarian cancer (EOC). Previous studies show cellular clusters in ascites of EOC patients are enriched with disseminated ovarian tumor cells that exhibit invasive capability in vitro, and that cell lines derived from these cells are tumorigenic. However, little is known about the tumorigenic and metastatic capabilities of these ovarian tumor cells. To initiate the investigation into the metastatic progression in ovarian cancer, tumor cells enriched in clusters of ascites of EOC patients were isolated and used to generate xenografts in NOD-SCID mice. Ovarian tumor cells were tagged with green fluorescence protein (GFP) using lentivirus to track their path during metastastic progression in the mice. Judging from the increased GFP signal in ascites and peritoneal organ metastases, the ovarian cancer xenografts established in NOD-SCID mice can be useful in the investigation on the molecular mechanism of metastatic progression in ovarian cancer.
Recommended Citation
Cho, Leong Chuen, "Approaches Toward Investigation of Metastatic Progression of Ovarian Tumor Cells" (2007). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 11.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/11