Type
Text
Type
Dissertation
Advisor
Zador, Anthony M. | Nancy Hollingsworth | Turner, Glenn | Deborah Brown | Neta Dean | Michael Frohman | Wei Guo
Date
2010-12-01
Keywords
centriole, prospore membrane, Sec2, SNARE, spindle pole body | Molecular Biology -- Cellular Biology
Department
Department of Molecular and Cellular Biology
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/70901
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
This research focuses on the production of new plasma membranes during gametogenesis in yeast. The initiation of these membranes is started by vesicle fusion at spindle pole bodies (SPBs) during Meiosis II. I have asked how these vesicles specifically fuse at SPBs but not at other locations, and demonstrated that binding interactions between soluble NSF attachment protein receptors (SNAREs) confer one layer of specificity to vesicle fusion at SPBs. Furthermore, I showed that SPBs can actively regulate a Rab GTPase, suggesting a role for SPBs in vesicle docking. My work highlights the ancestral dual roles of centrioles in microtubule organization and membrane nucleation, the latter of which is required for cilium formation in mammalian cells.
Recommended Citation
Yang, Hui-Ju, "The Specificity of Vesicle Trafficking during Prospore Membrane Formation in Yeast" (2010). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 109.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/109