Authors

Johnna St Clair

Type

Text

Type

Thesis

Advisor

Bogenhagen, Daniel , Garcia-Diaz, Miguel

Date

2012-12-01

Keywords

Molecular biology--Cellular biology--Biochemistry | Apoptosis, BH3-like Domain, FASTKD4, FAST Kinase, Mitochondria, TBRG4

Department

Department of Biochemistry and Cell Biology

Language

en_US

Source

This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.

Identifier

http://hdl.handle.net/11401/71040

Publisher

The Graduate School, Stony Brook University: Stony Brook, NY.

Format

application/pdf

Abstract

Mitochondria play an essential role in eukaryotic cells, supplying energy levels which vastly exceed those available to prokaryotes. They rely heavily on the import of nuclear encoded proteins for their function. The FAST family of proteins which are synthesized in the cytoplasm and imported into mitochondria are an example of this. The FAST family has not been well characterized, and next to nothing is known about several FAST family members. In the following I have reviewed what is known about each member of the FAST protein family including conserved domains, structures, including roles in cellular function, apoptosis and disease. Additionally, our lab has begun to characterize FASTKD4, one of the least studied FAST family members. We have determined that FASTKD4 is both a cytoplasmic and a mitochondrial protein, it does not reside in the outer mitochondrial membrane, its overexpression is not acutely toxic, and preliminary results suggest that it is not a member of any large complex in resting cells. | 65 pages

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