Authors

Wen Chen

Type

Text

Type

Thesis

Advisor

Kathlyn A. Parker. . | Iwao Ojima. Frank W. Fowler.

Date

2011-05-01

Keywords

β-Amino Acid Synthesis, β-lactams, epimerization, Fluorine, Schiff base, trans β-lactams | Chemistry

Department

Department of Chemistry

Language

en_US

Source

This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.

Identifier

http://hdl.handle.net/11401/71577

Publisher

The Graduate School, Stony Brook University: Stony Brook, NY.

Format

application/pdf

Abstract

β-Lactams are four-membered cyclic amides which can be found in many biologically active compounds. β-Lactams may possess two stereocenters. In enantiopure form, β-lactams can be used as chiral building blocks for the synthesis of various biologically relevant compounds, such as antibacterial drugs, antitumor agents, peptides, peptidomimetics and enzyme inhibitors. In addition, fluorine-containing β-lactams have drawn substantial interests among bioorganic and medicinal chemists. Studies have shown that the incorporation of fluorine efficiently blocks metabolism of the drug molecules, especially by cytochrome P-450 family of enzymes, providing improved pharmacokinetic properties. Different diastereomers of fluorine-containing β-lactams with high enantiopurity were synthesized utilizing a base-catalyzed C4-specific epimerization. Highly enantioenriched cis-β-lactams were obtained using the Staudinger [2+2] ketene-imine cycloaddition reaction, followed by kinetic enzymatic resolution. Ozonolysis of an exocyclic double bond of the enantioenriched cis-β-lactam afforded the key intermediate for selective epimerization, i.e. | a C4-formyl-β-lactam. The epimerization reaction was optimized for the isolation of trans-β-lactams. A difluorovinyl group was incorporated through a Wittig reaction, and subsequent modification of the substituents gave the desired 4-difluorovinyl-β-lactams. Using this protocol, a novel trans-difluorovinyl-β-lactam, (3S,4S)-1-(tert-butoxycarbonyl)-3-triisopropylsiloxy-4-(2,2-difluoroethenyl)azetidin-2-one with excellent enantiopurity, was obtained. These novel cis- and trans-fluorine-containing β-lactams can be used for the synthesis of fluorine-containing α-hydroxy-β-amino acids and their congeners or for the incorporation of fluorines into biologically active compounds via the β-lactam synthon method to investigate the effects of the fluorine moieties on their biological activities.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.