Type
Text
Type
Thesis
Advisor
Meng, Yizhi | Gersappe, Dilip | Qin, Yi-Xian.
Date
2011-12-01
Keywords
Materials Science
Department
Department of Materials Science and Engineering
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/71331
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Osteoporosis, a bone disease caused by the decreased mass and impaired structure of bone, is becoming an increasing threat to the health of the elderly population. To treat and prevent this kind of suffering, understanding of the bone formation and regeneration is of significant importance, during which bone cell-biomaterial interaction plays a critical role, especially osteoblasts. In this work, we report an in situ investigation of osteoblast proliferation, migration and differentiation on a series of substrates with variable surface chemistry as a result of coating fabrication. Primary investigation indicated that cells migrated faster on sulfonated polystyrene (SPS)-coated MirrIR slides than uncoated slides but grew slower. Cell elongation results were well consistent with the migration data. To explain these differences, hydrophilicity of SPS-coated and uncoated slides was measured, which turned out that SPS coating made the slides more hydrophobic. However, to successfully explain the results one challenge is that multiple discrepancies co-exist between SPS-coated and uncoated MirrIR slides, excluding surface chemistry, surface roughness and surface charge, which requires future work focusing on realization of univariate parameter at one time. Final understanding of effects of individual parameter listed above on osteoblast proliferation, migration and differentiation is hopeful to help design new implantable materials for clinical surgery. | 59 pages
Recommended Citation
Lu, Xia, "Investigation of Materials Surface Chemistry on Osteoblast Proliferation, Migration and Differentiation" (2011). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 537.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/537