Type
Text
Type
Thesis
Advisor
Futcher, Bruce | Neiman, Aaron
Date
2015-08-01
Keywords
budding yeast, meiosis, Rim4, RNA binding protein, translational regulation | Biochemistry
Department
Department of Genetics.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/77636
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
In Saccharomyces cerevisiae, translation is regulated in meiosis 1. Rim4 is an RNA binding protein with two RNA recognition motifs (RRMs). Previous studies 2 have shown that point mutations in either RRM knock out the translational repression of CLB3, SPO20, GIP1 and SPS1 in meiosis I, suggesting that the translational control of these genes may be due to Rim4. We wished to see the full range of RNAs bound by Rim4. We applied RIP-chip (RNA Immunoprecipitation/microarray) and CLIP-seq (in vivo Crosslinking and Immunoprecipitation/sequencing) to identify the RNAs co-immunoprecipitated with Rim4 in a synchronized meiosis I culture. The translationally-repressed genes co-clustering with CLB3, and translationally-repressed genes co-clustering with AMA1 in ribosome footprint data 1 were enriched in Rim4 immunoprecipitates compared to the negative controls. Therefore, these two groups of translationally repressed genes seem to be the targets of Rim4. Some other genes without translational repression in meiosis I were also enriched in Rim4 immunoprecipitates. | 50 pages
Recommended Citation
Xiao, Che, "Exploring RNA Binding Pattern of Rim4 in Meiosis" (2015). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 3431.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/3431