Type
Text
Type
Thesis
Advisor
Seeliger, Jessica | Sampson, Nicole S | Laughlin, Scott.
Date
2015-12-01
Keywords
Chemistry
Department
Department of Chemistry.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/77098
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Tuberculosis (TB) is one of the major causes of mortality in the world, causing approximately 1.5 million deaths every year, among which children take a great part. However, due to the perception that children are rarely infectious or they rarely develop severe disease, children with TB have always been neglected. What is worse, diagnosis of childhood TB is difficult, making it harder to control the disease. Foamy macrophages are a key component in the pathology of TB, and the formation of foamy macrophages foresees the progression of infection to active disease. Low-density lipoprotein (LDL) is one of the major lipoproteins that contain cholesterol, and important metabolism of cholesterol enables bacterial survival. After modification in the host, LDL could lead to foamy macrophage formation in the tubercular granuloma. We hypothesized that the modified LDL in patients with TB which induces foamy macrophage formation is different from other forms of LDL. Therefore, modified LDL in patients with TB could serve as a biomarker for TB diagnosis. Our long-term objective is to characterize modified LDL in TB patients and investigate its function in foamy macrophage formation. In this work, we first prepare Mtb-modified LDL, and then we set up macrophage experiment to investigate its function towards foamy macrophage formation. Then we focus on the characterization of Mtb-modified LDL using agarose gel electrophoresis, Western blot analysis, and MALDI-TOF analysis. | 65 pages
Recommended Citation
Chen, Guannan, "Modified low-density lipoprotein from Mycobacterium tuberculosis induces foamy macrophage formation" (2015). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2935.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2935