Type
Text
Type
Thesis
Advisor
Zhang, Lingbo | Dean, Neta | Stenlund, Arne.
Date
2015-12-01
Keywords
Biochemistry
Department
Department of Biochemistry and Cell Biology.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76906
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
The genetic underpinnings of a cancer are key to understanding the etiology of the disease and thus to providing a cure, because any robust cure of the disease will need to target a therapeutic window, a gene or a group of genes that are different between the cancerous cells and the normal cells. The research shown in this thesis gives insight into the genetic causes of acute myelogenous leukemia in an attempt to find a therapeutic window for this disease. A genetic screen using CRISPR-Cas9 is designed and conducted in a cell line designated RN 2-5 that was determined to recapitulate the acute myelogenous leukemia phenotype in culture and in a mouse model of the disease. Following up on the most important hits of this screen will very likely lead to important therapeutic targets. Two bioinformatics methods, a machine-learning algorithm and a statistical approach, are also used to identify key features of the expression of genes targeted in the screen. A combination of genetic screening and bioinformatics will have great efficacy in the future in finding genes differentially expressed, and differentially required, by cancer cells versus normal cells. | 43 pages
Recommended Citation
Chiappone, Sam Blake, "A Targeted CRISPR-Cas9 Screen in AML Cells" (2015). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2780.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2780