Type
Text
Type
Thesis
Advisor
Hollingsworth, Nancy M | Luk, Ed.
Date
2017-05-01
Keywords
Cellular biology | homologous recombination, srs2, zip1
Department
Department of Biochemistry and Cell Biology
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76890
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
Homologous Recombination (HR) is an essential mechanism for double strand break (DSB) repair that generates crossovers (COs). In meiosis, HR is required for proper chromosome segregation at the first meiotic divison. The Sgs1 helicase promotes the formation of non-crossovers (NCOs) through a process called synthesis-dependent strand annealing. A set of meiosis specific genes called the ZMM genes, protects strand invasion intermediates from Sgs1, resulting in the formation of a specific class of COs that are distributed throughout the genome. The transverse filament protein, Zip1 is encoded by one of the ZMM genes, and phosphorylation of Zip1 on four adjacent serines in its C terminus are required for the ZMM pathway of recombination. Previous work has shown the absence of SGS1 combined with a nonphosphorylatable version of ZIP1, zip1-4A, triggers the meiotic recombination checkpoint, resulting in meiotic prophase arrest. Srs2 is a less well studied helicase in meiosis, which like Sgs1, exhibits an anti-recombinase function during DSB repair in vegetative cells. The goal of my thesis was to see whether depletion of SRS2 has a similar genetic interaction in zip1-4A, which would suggest a role for SRS2 during meiosis similar to SGS1. | 53 pages
Recommended Citation
Joseph, Dimitri, "Characterization of a genetic interaction between the Srs2 helicase and phosphorylated Zip1 during budding yeast meiosis" (2017). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2764.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2764