Type
Text
Type
Thesis
Advisor
Rafailovich, Miriam | Sokolov, Jonathan | Gersappe, Dilip.
Date
2013-12-01
Keywords
cell culture, hydrogel, infectivity | Materials Science
Department
Department of Materials Science and Engineering.
Language
en_US
Source
This work is sponsored by the Stony Brook University Graduate School in compliance with the requirements for completion of degree.
Identifier
http://hdl.handle.net/11401/76362
Publisher
The Graduate School, Stony Brook University: Stony Brook, NY.
Format
application/pdf
Abstract
The interaction of cells with their extracellular matrix is of great importance when cells adapt to their environment. The purpose of this thesis is to design substrates with controllable properties and to study cellular interaction on these substrates. Firstly, enzymatically cross-linked gelatin hydrogels with different elastic modulus were prepared. Then, we studied the condition of cell growth and virus infection on these hydrogels as a followed-up. In the first part of the study, we made enzymatically cross-linked gelatin hydrogels with five different elastic modulus. As a parameter of stiffness, elastic modulus varies from 2.4 KPa to 7.5 KPa based on which hydrogels are graded from soft to hard. In the second part, we studied the growth of rabbit kidney cells cultured on hydrogels of different stiffness. Growth curves of the cells were made to study the abilities of soft and hard hydrogels to support cell proliferation. Result shows that cell proliferation rate differs when using hydrogel substrates of different stiffness as substrates. In the third part of this thesis, we infected rabbit kidney cells with pseudorabies virus for a period of time . And confocal fluorescence microscopy was used to investigate the influence of hydrogels with different elastic modulus on infectivity of the virus. | 49 pages
Recommended Citation
Yang, Fan, "Influence of Hydrogel Substrate on Cell Growth and Virus Infection" (2013). Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions). 2286.
https://commons.library.stonybrook.edu/stony-brook-theses-and-dissertations-collection/2286